Structure of Microbial Communities When Complementary Effluents Are Anaerobically Digested

Olive oil and pig productions are important industries in Portugal that generate large volumes of wastewater with high organic load toxicity, raising environmental concerns. The principal objective this study is to energetically valorize these effluents—piggery effluent olive mill wastewater—through the anaerobic digestion biogas/methane production, by means complementarity concept. Several mixtures piggery were tested, an increasing percentage wastewater. best performance was obtained for samples alone admixture 30% OMW, which provided same volume biogas (0.8 L, 70% CH4), 63/75% COD removal, 434/489 L CH4/kg SVin, respectively. validation process assessed molecular evaluation through Next Generation Sequencing (NGS) 16S rRNA gene. structure microbial communities both samples, throughout process, characterized predominance bacterial populations belonging phylum Firmicutes, mainly Clostridiales, Bacteroidetes being subdominant populations. Archaea genus Methanosarcina became predominant digestion, confirming formation methane from acetate, line greatest removal volatile fatty acids (VFAs) samples.